Taq Buffer
Summary
10x buffer for PCR with Taq. Makes 1mL.
Ingredients
10x buffer for PCR with Taq. Makes 1mL.
Ingredients
- 100 μL 1M Tris-HCl pH 8.3
- 167 μL 3M KCl
- 733 μL H2O
- Mix, vortex.
- Store at -20°C
- Should be supplemented with MgCl2 when used. A working concentration of 2.5mM (a 1/20 dilution of 50mM stock) works well for most applications.
- 3/2016: YHW says 1.6mM is the appropriate amount of MgCl2